Janus Green B

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Janus Green B[1]
Janus green B.svg
Ball-and-stick model of the component ions of Janus Green B
Janus Green B powder.jpg
Solid Janus Green B
Janus Green B solution.jpg
Janus Green B Solution
IUPAC name
8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
Other names
Diazin Green S
Union Green B
3D model (JSmol)
ECHA InfoCard 100.018.814 Edit this at Wikidata
EC Number
  • 220-695-6
  • InChI=1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1 checkY
  • CCN(CC)C1=CC2=[N+](C3=C(C=CC(=C3)N=NC4=CC=C(C=C4)N(C)C)N=C2C=C1)C5=CC=CC=C5.[Cl-]
Molar mass 511.06 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
☒N verify (what is checkY☒N ?)
Infobox references

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.


  1. ^ Janus Green B, at Sigma-Aldrich
  2. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
  3. ^ "Archived copy". Archived from the original on 2006-02-24. Retrieved 2007-10-31.CS1 maint: archived copy as title (link)